10 Magnification Error
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What Is Magnification In Microscope
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What Is Magnification In Physics
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Overview Keeping a lab notebook Writing research papers Dimensions & units Using figures (graphs) Examples of graphs Experimental error Representing error Applying statistics Overview Principles of microscopy Solutions & dilutions Protein assays Spectrophotometry Fractionation & centrifugation Radioisotopes and detection overview; types; bright field microscopy dark field optics phase contrast oil immersion
Camera Lens Magnification Chart
differential interference contrast measuring using a counting chamber wet (Vaseline) mount Measurement with contrast the way light microscopes and electron microscopes magnify objects the Light Microscope Your microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule can be
Lens Magnification Ratio
used to measure any planar dimension in a microscope field since the ocular can be turned in any direction and the object of interest can be repositioned with the stage manipulators. To measure the length of an object http://www.ncbi.nlm.nih.gov/pubmed/9030472 note the number of ocular divisions spanned by the object. Then multiply by the conversion factor for the magnification used. The conversion factor is different at each magnification. Therefore, when using a reticule for the first time, it is necessary to calibrate the scale by focusing on a second micrometer scale (a stage micrometer) placed directly on the stage. Conversion factor Identify the ocular micrometer. A typical scale consists of 50 - 100 divisions. You may have to http://www.ruf.rice.edu/~bioslabs/methods/microscopy/measuring.html adjust the focus of your eyepiece in order to make the scale as sharp as possible. If you do that, also adjust the other eyepiece to match the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched with divisions of 0.01 mm (10 µm). Suppose that a stage micrometer scale has divisions that are equal to 0.1 mm, which is 100 micrometers (µm). Suppose that the scale is lined up with the ocular scale, and at 100x it is observed that each micrometer division covers the same distance as 10 ocular divisions. Then one ocular division (smallest increment on the scale) = 10 µm at 100 power. The conversion to other magnifications is accomplished by factoring in the difference in magnification. In the example, the calibration would be 25 µm at 40x, 2.5 µm at 400x, and 1 µm at 1000x. Some stage micrometers are finely divided only at one end. These are particularly useful for determining the diameter of a microscope field. One of the larger divisions is positioned at one edge of the field of view, so that the fine part of the scale ovelaps the opposite side. The field diameter can t
article Abstract INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION Acknowledgements http://onlinelibrary.wiley.com/doi/10.1359/jbmr.1997.12.1.119/full References Related Content Citing Literature Journal of Bone and Mineral Research Explore this journal > Explore this journal > Previous article in issue: Correlation of Trabecular Bone Structure with Age, Bone Mineral Density, and Osteoporotic Status: In Vivo Studies in the Distal Radius Using High Resolution Magnetic what is Resonance Imaging Previous article in issue: Correlation of Trabecular Bone Structure with Age, Bone Mineral Density, and Osteoporotic Status: In Vivo Studies in the Distal Radius Using High Resolution Magnetic Resonance Imaging Next article in issue: Sources of Variability in Bone Mineral Density Measurements: Implications for Study Design what is magnification and Analysis of Bone Loss Next article in issue: Sources of Variability in Bone Mineral Density Measurements: Implications for Study Design and Analysis of Bone Loss View issue TOC Volume 12, Issue 1 January 1997 Pages 119–123 ArticleCorrecting the Magnification Error of Fan Beam DensitometersAuthorsM. R. Griffiths,Department of Nuclear Medicine and Bone Densitometry St. Vincent's Hospital, Sydney, AustraliaSearch for more papers by this authorK. A. Noakes,Department of Nuclear Medicine and Bone Densitometry St. Vincent's Hospital, Sydney, AustraliaSearch for more papers by this authorN. A. Pocock M.B., B.S., M.D., FRACPCorresponding authorDepartment of Nuclear Medicine and Bone Densitometry St. Vincent's Hospital, Sydney, AustraliaDepartment of Nuclear Medicine and Bone Densitometry, St. Vincent's Hospital, Sydney, NSW 2010, AustraliaSearch for more papers by this authorFirst published: 1 January 1997Full publication historyDOI: 10.1359/jbmr.1997.12.1.119View/save citationCited by: 28 articles Citation tools Set citation alert Check for new citations Citing lite
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