Pcr And Error Bar
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Error Bars For Normalized Data
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group with error bars on the relative expression histograms in a qPCR study. I have a question regarding to the representation of the relative expression histograms. We did a qpcr error bar calculation comparative qPCR study, and, using the Pfaffl's efficiency corrected Ct formula, I calculated
Qpcr Fold Change Standard Deviation
the relative expression values for all of my samples. Since I have biological replicates, I get error bars for
Qpcr Error Propagation
the treatment groups on the relative expression values, but in many papers I noticed that they also use error bars for the control groups (value 1 with an error bar). How? Topics https://www.reddit.com/r/labrats/comments/2jkgpp/error_bars_for_qpcrrtpcr/ PCR × 5,016 Questions 71,924 Followers Follow Real-Time PCR × 2,151 Questions 3,370 Followers Follow Feb 12, 2013 Share Facebook Twitter LinkedIn Google+ 2 / 0 All Answers (3) Jo Vandesompele · Ghent University Biogazelle's qbasePLUS software (http://www.qbaseplus.com) can do this. If you want to do this manually in a spreadsheet, I would need a bit more information on how EXACTLY you did you https://www.researchgate.net/post/How_to_represent_control_group_with_error_bars_on_the_relative_expression_histograms_in_a_qPCR_study calculations. If you calculated relative quantities for all you samples at once (e.g. according to Hellemans et al., Genome Biology, 2007), you will also have variable results for your control group and hence an error bar for this group. Feb 13, 2013 Jack M Gallup · Iowa State University Dr. V, your software is the best in the world for this. It's good to let the cat out of the bag at this point. And it is always good to hear your opinion on qPCR stats. The error bars for the controls is always propotional to their error bars before the control was divided by itself. I believe this is also equal to the coefficient of variance. E.g., if the error bar for a control value of 0.5 was +/- 0.2 (before control was divided by itself), then, when the control becomes "1" by self-division, the error bar becomes (by proportion or coefficient of variance rules) +/- 0.4. But if the error bars are the result of transformation from log to linear scale, the error bar above and below the median is not symmetrical... technically, and thus a more lengthy explanati
biological replicate - (Dec/20/2011 )Visit this topic http://www.protocol-online.org/biology-forums-2/posts/23815.html in live forum Printer Friendly VersionABI has a step by step guide for qPCR statistics that ultimately gives you the fold change and the standard deviation (can be found here). My assumption is that the calculated SD only represents the variation in error bar technical replicates and not the biological replicates. How would you incorporate the variations in both technical and biological replicates into the final error bars (say SD)? Is the technical replicate SD is reflected in the final result at all? any input is pcr and error highly appreciated. Kaveh -kaveh- From what I have seen, most people just plot technical replicates +/- error bars (95% CIs) and then they indicate that they repeated the experiment 2 or 3 times and had similar results. I think for biological replicates you are correct in assuming that you just ignore the SD for the technical replicates and just calculate the mean and SD for the biological replicates. -doxorubicin- Yes, we take SD of technical replicates as a indication how precise was the experiment, but if we plott biological replicates, we only calculate SD for the biological ones and ignore the technical. -Trof- Visit this topic in BioForum Printer Friendly Version About Terms of Service Privacy Feedback Sponsorship © 1999-2013 Protocol Online, All rights reserved.