Pcr Polymerase Error Rate
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4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer as reported by Finnzymes/Thermo taq polymerase proofreading Scientific. Links to this resource Products: Phusion High-Fidelity PCR Master Mix
Polymerase Error Rate Comparison
with GC Buffer, Phusion High-Fidelity DNA Polymerase, Phusion High-Fidelity PCR Master Mix with HF Buffer, Phusion High-Fidelity PCR
Enzyme Fidelity Definition
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Processing Charges · Articles in Press · Author Guidelines · Bibliographic Information · Citations to this Journal · Contact Information · Editorial Board https://www.hindawi.com/journals/mbi/2014/287430/ · Editorial Workflow · Free eTOC Alerts · Publication Ethics · Reviewers Acknowledgment · Submit a Manuscript · Subscription Information · Table of Contents Open Special Issues http://genome.cshlp.org/content/3/3/S18.full.pdf · Published Special Issues · Special Issue Guidelines Abstract Full-Text PDF Full-Text HTML Full-Text ePUB Full-Text XML Linked References Citations to this Article How to Cite this Article error rate Views 13,721 Citations 20 ePub 31 PDF 2,285 Molecular Biology InternationalVolume 2014 (2014), Article ID 287430, 8 pageshttp://dx.doi.org/10.1155/2014/287430Research ArticleError Rate Comparison during Polymerase Chain Reaction by DNA PolymerasePeter McInerney,1,2 Paul Adams,1,3 and Masood Z. Hadi1,3,41Joint BioEnergy Institute, Emeryville, CA, USA2Sandia National Laboratories, Livermore, CA, USA3Physical Biosciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA4Synthetic Biology Program, Space BioSciences Division, NASA polymerase error rate AMES Research Center, Mail Stop 239-15, Moffett Field, CA 94035, USAReceived 22 May 2014; Accepted 21 July 2014; Published 17 August 2014Academic Editor: Alessandro Desideri Copyright © 2014 Peter McInerney et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractAs larger-scale cloning projects become more prevalent, there is an increasing need for comparisons among high fidelity DNA polymerases used for PCR amplification. All polymerases marketed for PCR applications are tested for fidelity properties (i.e., error rate determination) by vendors, and numerous literature reports have addressed PCR enzyme fidelity. Nonetheless, it is often difficult to make direct comparisons among different enzymes due to numerous methodological and analytical differences from study to study. We have measured the error rates for 6 DNA polymerases commonly used in PCR applications, including 3 polymerases typically used for cloning applications requiring high fidelity. Error rate measurement values reported here were