Phusion Error Rate
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4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer as reported by Finnzymes/Thermo Scientific. Links to
Taq Polymerase Error Rate
this resource Products: Phusion High-Fidelity PCR Master Mix with GC Buffer, Phusion pcr error rate calculator High-Fidelity DNA Polymerase, Phusion High-Fidelity PCR Master Mix with HF Buffer, Phusion High-Fidelity PCR Kit Products Product CatalogNew polymerase error rate comparison ProductsSpecial Offers My NEB Products Save your favorite products by clicking Add to My NEB, making re-ordering and remembering what you need quick and simple. Tools & Resources Returning to use
Phusion High-fidelity Dna Polymerase
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Pcr Fidelity Calculator
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units2,000 units/ml$106.00Quantity: Add to Cart M0530L500 units2,000 units/ml$424.00Quantity: Add to Cart   phusion vs q5 Categories: Phusion® High-Fidelity DNA Polymerase Products Applications: DNA Blunting, Fast PCR,
Dna Polymerase Error Rate
Gibson Assembly®, High-Fidelity PCR, Long Range PCR, Multiplex PCR, Routine PCR, Specialty PCR ProductInformationFAQs phusion tm calculator &Tech TipsProtocols &ManualsOther Tools & ResourcesQuality &SafetyLegalInformation DescriptionAdvantages and FeaturesProperties and UsageRelated ProductsReferences Description High-Fidelity DNA Polymerases are important for applications in which https://www.neb.com/faqs/2012/09/06/what-is-the-error-rate-of-phusion-reg-high-fidelity-dna-polymerase the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused https://www.neb.com/products/m0530-phusion-high-fidelity-dna-polymerase with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl2 solutions. Fidelity assays were performed using a lacI-based meth
$0.97 link Phusion HF The following table lists a variety of polymerases, some immediately available in lab and some not, and useful information http://barricklab.org/twiki/bin/view/Lab/ProtocolsTaq about each of them. Taq Platinum Taq KOD PFU Vent Phusion Species Thermophilis Aquaticus Mix of Taq, Pyrococcus species GB-D polymerase and Platinum® Taq Antibody Thermococcus kodakaraensis Pyrococcus furiosis Thermococcus litoralis Pyrococcus + Processivity domain Fidelity (err/bp) 10–4 to 10–5 10–5 to 10–6 2.6x10–6 1-3 x 10–6 10–6 10–6 to 10–7 Elongation Rate 1 min/kb 1 min/kb 20-30 sec/kb 1 min/kb error rate 1 min/kb 20-30 sec/kb Processivity ~ 50 bases > 50 bases >300 bases 20 ? 35 Nicking Activity None None None ? < 10% ? Amplicon Size < 5kbp Up to 20 kbp Plasmid/Linear = 6kbp Genome = 2kbp Vector: Up to 15 kbp Genome = Up to 19 kbp ? Genome = 10 kbp Plasmid/Linear = 20 kbp 3' → polymerase error rate 5' exonuclease? (proofreading) No Yes (Pyrococcus) Yes Yes Yes Yes 5' → 3' exonuclease? Yes Yes No No No No Strand displacing? Yes Yes No No Yes No Overhang? 3' - A 3' - A No No No No GC-Rich Performance Low Low High Medium High Medium Hot Start? No Yes Available Available No Available Applications Standard Taq Diagnostic PCR Since Taq is the cheapest polymerase available, it is the most appropriate for diagnostic applications requiring multiple reactions, such as checking if your cloning or genomic insertion worked via PCR of the intended insert region. Colony PCR Taq is especially well-suited to PCR on the unpurified genome, as in colony PCR. Taq will more successfully (and less expensively) amplify a single product off a small number of cells than Phusion. Phusion High Fidelity High-fidelity PCR The error rate of Phusion DNA Polymerase is 4.4 x 10^-7; therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy. Cloning Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choi
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