Polymerase Error Rate Calculation
Contents |
Go phusion polymerase error rate Applications & TechniquesMost Popular Products Lab SolutionsChemicalsLab
Dna Polymerase Fidelity
AutomationLab Data Management & Analysis SoftwareLab EquipmentLab Plasticware & SuppliesBrandsThermo Scientific™Applied dna polymerase error rate Biosystems™Invitrogen™Gibco™Ion Torrent™Life SciencesAntibodiesCell AnalysisCell Culture & TransfectionCloning | Synthetic BiologyDNA & RNA PurificationEpigeneticsFlow CytometryGene Expression AnalysisGenome EditingPCR pcr fidelity calculator | Real-time PCRProtein BiologyRNAiSequencingStem Cell ResearchIndustrial & Applied ScienceAgricultural BiotechnologyAnimal HealthBioproductionCement, Coal & MineralsChromatographyEnvironmentalFood & BeverageForensicsManufacturing & ProcessingMass SpectrometryMicrobiologyPharma & BiopharmaRadiation Detection & MeasurementSpectroscopy, Elemental & Isotope AnalysisClinical & DiagnosticsAnatomical PathologyCancer ResearchClinical MicrobiologyClinical & Translational ResearchDiagnostic
Polymerase Error Rate Comparison
DevelopmentDiagnostic TestingPreclinical to Companion Diagnostic DevelopmentPublic HealthProduct Selection GuidesServicesNew ProductsPromotionsLearning CentersShop All Products Most Popular ProductsBeakersLab CentrifugesUltra Low–Temperature FreezersLipofectamine™ ReagentsNalgene™ LabwareOligos, Primers, Probes & NucleotidesPipettes & Pipette TipsSuperScript™ Reverse TranscriptaseTaqMan® Real-Time PCR AssaysTRIzol™ ReagentsMost Popular CategoriesAntibodies & ImmunoassaysCell Culture & Transfection ReagentsChromatography Columns, Resins, & Spin FiltersDrug Discovery AssaysGels & MembranesInstruments & EquipmentLab Reagents & ChemicalsLab Supplies, Plastics & GlasswarePrimers/Oligos, Cloning & Gene SynthesisSoftware & Digital StorageOrder ToolsHow to OrderQuick OrderTrack Your OrderOrder HistoryOrder SupportContact UsShop All ProductsShop All ServicesProduct Selection GuidesServices & SupportProduct DocumentationCertificates of Analysis and ConformanceChemical StructuresCitations & ReferencesManufacturing Site ISO CertificationsManualsMedia FormulationsNewsletters & JournalsProduct LiteratureRoHS CertificatesSDSsSpectral DataSupplemental ProtocolsVector MapsServicesCustom Services
Processing Charges · Articles in Press · Author Guidelines · Bibliographic rna polymerase error rate Information · Citations to this Journal · Contact dna polymerase fidelity comparison Information · Editorial Board · Editorial Workflow · Free eTOC Alerts · Publication Ethics
Gotaq Error Rate
· Reviewers Acknowledgment · Submit a Manuscript · Subscription Information · Table of Contents Open Special Issues · Published Special Issues https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/pcr-fidelity-calculator.html · Special Issue Guidelines Abstract Full-Text PDF Full-Text HTML Full-Text ePUB Full-Text XML Linked References Citations to this Article How to Cite this Article Views 13,729 Citations 20 ePub 31 PDF 2,287 Molecular Biology InternationalVolume 2014 (2014), Article ID 287430, 8 pageshttp://dx.doi.org/10.1155/2014/287430Research ArticleError Rate Comparison during Polymerase https://www.hindawi.com/journals/mbi/2014/287430/ Chain Reaction by DNA PolymerasePeter McInerney,1,2 Paul Adams,1,3 and Masood Z. Hadi1,3,41Joint BioEnergy Institute, Emeryville, CA, USA2Sandia National Laboratories, Livermore, CA, USA3Physical Biosciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA4Synthetic Biology Program, Space BioSciences Division, NASA AMES Research Center, Mail Stop 239-15, Moffett Field, CA 94035, USAReceived 22 May 2014; Accepted 21 July 2014; Published 17 August 2014Academic Editor: Alessandro Desideri Copyright © 2014 Peter McInerney et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractAs larger-scale cloning projects become more prevalent, there is an increasing need for comparisons among high fidelity DNA polymerases used for PCR amplification. All polymerases marketed for PCR applications are tested for fidelity properties
faq • rss Community Log In Sign Up Add New Post Question: Simulate The Error Of Whole Pcr Process 0 2.7 years ago by J.F.Jiang • 620 China J.F.Jiang • 620 wrote: Hi all, I just want to simulate the whole https://www.biostars.org/p/93657/ PCR process to calculate the errors. Suppose that we have 1000 same DNA templates, and we will do 30 loops to amplify the DNA molecules. In each round, we set the 90% of the total DNA molecules will be amplified, and for each site, we suppose that the nucleotide has a 0.1% mismatch. And after the whole process, we want to take a look at the rate of changes at each site, compare to the original templates. If error rate there is any one who knows the existed tool, can you please give a hint? Thanks pcr • 1.4k views ADD COMMENT • link • Not following Follow via messages Follow via email Do not follow modified 2.7 years ago by mikhail.shugay ♦ 2.9k • written 2.7 years ago by J.F.Jiang • 620 1 2.7 years ago by Neilfws ♦ 46k Sydney, Australia Neilfws ♦ 46k wrote: A quick web search for the term "pcr error simulation" reveals polymerase error rate Estimation of the mutation rate during error-prone polymerase chain reaction and several other publications. ADD COMMENT • link written 2.7 years ago by Neilfws ♦ 46k 0 2.7 years ago by mikhail.shugay ♦ 2.9k Czech Republic, Brno, CEITEC mikhail.shugay ♦ 2.9k wrote: This is quite a complex question. Do you simply need the number of molecules with single (double, etc) mismatch difference from parent? Then have a look at this manuscript http://e-collection.library.ethz.ch/eserv/eth:1397/eth-1397-01.pdf, especially section 1.2.3, where lambda is PCR efficiency (0.9 in your case), G is segment length, mu is error rate per base per cycle and n is the number of cycles. In this case you can just use analytic solution. Of course if you need to look for each site directly, i.e. you need to simulate site-specific errors (and PCR errors are quite biased in this way, check e.g. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0070388), then a custom software is needed. Unfortunately, I havent come across any such software. PS A simple calculator is also available here http://www.thermoscientificbio.com/webtools/fidelity/ Hope this helps, Mike ADD COMMENT • link written 2.7 years ago by mikhail.shugay ♦ 2.9k yes, your suggestions are very helpful. I meant to write a script to manipulate the PCR process, however, when the total molecules after amplification are larger than 1e8, randomly select the position among the reads becomes very hard. If there any method to quick index the numbers? such as, if I have