Growth Curve Error Bars
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strains using an automated spectrophotometer (Bioscreen C MBR) that automatically records the OD600 from a 100-wells microtiter plate every 15 min for 4 days. I have triplicates for each strain. I use Excel to create a scatter plot of the OD vs how to make a graph with error bars in excel time. I can't figure out how can I perform some kind of statistical analysis on these how to add error bars in excel 2010 datas: should I calculate the SD between the three OD values for each time-frame? And if so, does that mean that I should have one how to add error bars in excel 2013 error bar for each time frame? (Maybe it's worth saying that I end up with a total of 384 values for each strain) Thank you all in advance Topics Biostatistics × 776 Questions 46,997 Followers Follow Statistical Data Analysis × 675 how to add individual error bars in excel Questions 879 Followers Follow Spectrophotometry × 467 Questions 372 Followers Follow Biostatistical Methods × 631 Questions 2,997 Followers Follow Standard Error × 120 Questions 11 Followers Follow Feb 1, 2015·Modified Feb 1, 2015 by the commenter. Share Facebook Twitter LinkedIn Google+ 0 / 0 All Answers (4) Jochen Wilhelm · Justus-Liebig-Universität Gießen What kind of "statistical analysis" are you talking about? I mean: you should have a particular question or problem, and then you can find a way to answer this question (or
Custom Error Bars Excel
solve the problem) with reasonable efficiency. Something like "I have some data" is not a formulation of a question/problem related to a statistical analysis. If your problem is just how to show the *data*, then there is not much required. Show all the triplicates. No need to calculate means and error bars. If you what to get a not-too-busy plot of the groth curves, then show (a line through) the averages of the triplicates and forget the error bars. If you want to *analyze* the data w.r.t. a growth model, then you should use the data to fit the model. You may then visualize the data (or the means of the triplicates) together with the fitted model curve, and indicate the uncertainty in the model by showing its confidence bands. Hint: if you aiming to analyze the differencese in the growth curves between different stains you might consider a mixed-effect model (with "culture" or "plate" as random effect). Unfortunately, Excel is out then. Feb 1, 2015 Deleted Hi and thanks for your reply. Yes you're right, I think I used the term "statistical analysis" inappropriately. My question was actually on a much more basic level. At this point I'm nowhere near doing any kind of advance modeling. What I really wanted to understand was when is appropriate to use SE and SD (correct me if I'm wrong but these in fact are, by definition, statistics) in the case of microbe growth experiments, where the data are represent
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What Are Error Bars
Video ist nicht verfügbar. WiedergabelisteWarteschlangeWiedergabelisteWarteschlange Alle entfernenBeenden Wird geladen... Wiedergabeliste Warteschlange __count__/__total__ how to add error bars in excel 2016 Adding unique standard error bars to a scattergraph in MS Excel 2011 Stephanie Castle AbonnierenAbonniertAbo beenden9.3519 Tsd. Wird geladen... how to calculate error bars Wird geladen... Wird verarbeitet... Hinzufügen Möchtest du dieses Video später noch einmal ansehen? Wenn du bei YouTube angemeldet bist, kannst du dieses Video zu einer Playlist hinzufügen. Anmelden Teilen Mehr https://www.researchgate.net/post/How_do_I_properly_use_error_bars_in_a_OD_vs_time_chart Melden Möchtest du dieses Video melden? Melde dich an, um unangemessene Inhalte zu melden. Anmelden Transkript Statistik 4.252 Aufrufe 9 Dieses Video gefällt dir? Melde dich bei YouTube an, damit dein Feedback gezählt wird. Anmelden 10 0 Dieses Video gefällt dir nicht? Melde dich bei YouTube an, damit dein Feedback gezählt wird. Anmelden 1 Wird geladen... Wird geladen... Transkript https://www.youtube.com/watch?v=QB_305Dg3RM Das interaktive Transkript konnte nicht geladen werden. Wird geladen... Wird geladen... Die Bewertungsfunktion ist nach Ausleihen des Videos verfügbar. Diese Funktion ist zurzeit nicht verfügbar. Bitte versuche es später erneut. Veröffentlicht am 31.08.2013Here I show you how to add unique standard error bars to a scattergraph. I have used MS Excel 2011 on a Mac. Kategorie Bildung Lizenz Standard-YouTube-Lizenz Mehr anzeigen Weniger anzeigen Wird geladen... Anzeige Autoplay Wenn Autoplay aktiviert ist, wird die Wiedergabe automatisch mit einem der aktuellen Videovorschläge fortgesetzt. Nächstes Video Excel: Graphing with separate Error Bars of Standard Deviation - Dauer: 6:38 Peter Stanley 14.656 Aufrufe 6:38 Excel Overview Lesson Two: Standard Deviation & Graphing with Error Bars - Dauer: 10:45 TheTopdog1957 1.941 Aufrufe 10:45 Add Error Bars to a Line Chart - Dauer: 4:18 Doug H 95.084 Aufrufe 4:18 How Do I Create Error Bars in Excel Charts? - Dauer: 7:05 Dr. Gerard Verschuuren 2.838 Aufrufe 7:05 Simple Custom Error Bars Excel 2013 - Dauer: 5:17 Jeff Muday 61.167 Aufrufe 5:17 IB Physics: Formulas, Trendlines and Error bars in Excel - Dauer: 11:2
place the https://www.graphpad.com/guides/prism/5/user-guide/reg_exponential_growth.htm first into column A, the second into https://www.graphpad.com/guides/prism/5/user-guide/using_replicates_and_error_bars_on_x.htm column B, etc. After entering data, click Analyze, choose nonlinear regression, choose the panel of exponential equations, and choose Exponential growth. Consider constraining Y0 to a constant value The parameter Y0 error bars is the Y value at time zero. In many cases, you will know this value precisely. If so, you should constrain that parameter to be a constant value. To do this, go to the Constrain tab of the nonlinear error bars in regression dialog, set the drop down next to Y0 to "Constant equal to" and enter its value. Model Y=Y0*exp(k*X) Y0 is the Y value when X (time) is zero. It is expressed in the same units as Y, K is the rate constant, expressed in reciprocal of the X axis time units. If X is in minutes, then K is expressed in inverse minutes. Tau is the time constant, expressed in the same units as the X axis. It is computed as the reciprocal of K. Doubling-time is in the time units of the X axis. It is computed as ln(2)/K. Copyright (c) 2007 GraphPad Software Inc. All rights reserved. URL: http://www.graphpad.com/help/Prism5/Prism5Help.html?reg_exponential_growth.htm
Setting up data tables for entry of replicates or error values When you create an XY table, choices on the Welcome or New Table dialog let you specify side-by-side subcolumns for entry of replicates or error values computed elsewhere. Learn more about XY tables. The example below is set for entry of two replicate values (duplicates) but you can choose any number from 2 to 52. This next example is set up to enter mean, SD and N for each point. "%CV" is the coefficient of variation, which is equals 100SD/Mean. If you enter the %CV, Prism will plot SD error bars. If you enter N along with the SD, SEM or %CV, choose to plot the SD, SEM, or 95% CI (via choices on the Format Graph dialog). If you omit N, Prism can only plot the error value you entered. Note that with XY and Grouped tables, replicates are entered into side-by-side subcolumns. With Column data, replicates are stacked into columns. Changing the subcolumn format Click the Table Format button in the upper left corner of the table to reformat a data table - change the number of replicates, or change the entry of error values. Use the Format Data Table dialog to specify how you will enter the replicates or error values. If you change from entry of mean and SD to entry of mean and SEM, only the labels change (not the numbers). This lets you correct a mistake (if you were actually entering SEM values but mistakenly set up the table with a subcolumn labeled for SD values). Don't make changes in the Format Data Table dialog when you want to change the way the error bars are plotted. Read on to see how to change error bar plotting. Choosing how the replicates or error bars are plotted When you create a new table and enter replicates, you choose not only how the subcolumns are formatted, but also how Prism plots them. You can choose to plot individual replicates, mean only, mean with error bar (which you can specify). Choose from a pair of drop down lists directly below the place where you choose the number of replicates. If you choose to enter error values directly, Prism will plot the values you entered (except that when you enter %CV, Prism plots the SD). If you entered Mean, SD (or SEM) and N, you can choose (on the graph) to plot the error bars as SD, SEM or 95% CI. Changing how replicates or error bars are plotted To plot the