Real Time Pcr Error Bars
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Fold Change Error Bars
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Qpcr Data Analysis Error Bars
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Error Bars For Normalized Data
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faq • rss Community Log In Sign Up Add New Post Question: Error Bars Following Normalisation Of Real Time Pcr Data 0 5.6 years ago by standard error qpcr Dolores Hamilton • 0 Dolores Hamilton • 0 wrote: Using the 2-delta delta
Qpcr Biological Replicates Standard Deviation
Ct method I have determined the relative gene expression in a panel of 7 treated and untreated cell lines-part qpcr fold change standard deviation of data shown below (C=untreated; T=treated): Sample RQ RQ RQ D556C 2.6588333 2.1409998 4.9944763 D556T 9.368949 7.3571334 18.470724 D384C 14.2748 10.199899 18.23931 D384T 11.659178 9.1894222 7.546831 I used Prism to represent https://www.reddit.com/r/labrats/comments/2jkgpp/error_bars_for_qpcrrtpcr/ the data as a bar chart with error bars For each cell line I want to look at the fold change in expression between the untreated and treated, and so for each replicate and cell line I normalised the treated to the untreated-part of data shown below: Sample Foldchange Foldchange Foldchange D556C 1 1 1 D556T 3.5 3.4 3.7 Using prism when I graphically https://www.biostars.org/p/7138/ represent the fold change data I get error bars for the treated cell line, how do I get error bars for the untreated cell line to which the treated has been normalised? i.e all untreated replicates have a value of 1. When I look in the literature results are displayed with error bars on the untreated 1 x sample? Many thanks Dolores data • 9.4k views ADD COMMENT • link • Not following Follow via messages Follow via email Do not follow modified 5.6 years ago by Chris Evelo ♦ 9.7k • written 5.6 years ago by Dolores Hamilton • 0 This is not very clear at all. Are you saying that there are 7 cell lines, each either treated or untreated? It would help if you could show some raw data and explain what software was used. It also sounds as though you have 2 observations per cell line (1 treated, 1 untreated), in which case error bars are quite meaningless. ADD REPLY • link written 5.6 years ago by Neilfws ♦ 46k 1 5.6 years ago by Chris Evelo ♦ 9.7k Maastricht, The Netherlands Chris Evelo
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