Calculate Error Bars Fold Change
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(or SEM) when using excel to calculate ddct method for real time PCR? We have been using the deltadelta CT to evaluate gene how to calculate fold change in qpcr expression - give or take its own intrinsic methods. We use our own how to calculate fold change from log2 excel sheet to calculate the fold expression. What is the best way to calculate the SD? Topics PCR
How To Calculate Fold Change From Microarray Data
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How To Calculate Fold Change Gene Expression
23, 2013 Share Facebook Twitter LinkedIn Google+ 1 / 0 Popular Answers Jochen Wilhelm · Justus-Liebig-Universität Gießen You can directly and simply calculate the SD or SEM of the delta-cts. Now you calculate the delta-delta-ct from the two average delta-cts (treated and control, for instance). The SD or SEM of this difference can be derived using Gauß' error propagation. When s is the how to calculate fold change in excel SD or SEM of the delta-ct's, then s' of the delta-deltas is given as s' = SQRT(s²[treat]+s²[control]). A more direct approach to the delta-delta-ct together with its SEM is to perform a two-way ANOVA with interaction on the delta-ct's. The interaction is then the delta-delta-ct. I know that it is a strong urge to convert the delta-delta-cts into fold-changes (by antilog). If you present fold-changes, however, then please do NOT report SD or SEM of this quantity. These statsitics are not instructive here, because the distribution is not anymore symmetric. To give an indication of the uncertainty/precision of the fold-changes, claculate the confidence interval for the delta-delta-ct and then antilog the limits to get the confidence limits for the fold-change. Sep 24, 2013 All Answers (5) Jochen Wilhelm · Justus-Liebig-Universität Gießen You can directly and simply calculate the SD or SEM of the delta-cts. Now you calculate the delta-delta-ct from the two average delta-cts (treated and control, for instance). The SD or SEM of this difference can be derived using Gauß' error propagation. When s is the SD or SEM of the delta-ct's, then s'
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How To Calculate Fold Change In Real Time Pcr
by Dolores Hamilton • 0 Dolores Hamilton • 0 wrote: Using the how to calculate error bars by hand 2-delta delta Ct method I have determined the relative gene expression in a panel of 7 treated and untreated how to calculate error bars in physics cell lines-part of data shown below (C=untreated; T=treated): Sample RQ RQ RQ D556C 2.6588333 2.1409998 4.9944763 D556T 9.368949 7.3571334 18.470724 D384C 14.2748 10.199899 18.23931 D384T 11.659178 9.1894222 7.546831 I used Prism https://www.researchgate.net/post/What_is_the_best_way_to_calculate_standard_deviation_or_SEM_when_using_excel_to_calculate_ddct_method_for_real_time_PCR to represent the data as a bar chart with error bars For each cell line I want to look at the fold change in expression between the untreated and treated, and so for each replicate and cell line I normalised the treated to the untreated-part of data shown below: Sample Foldchange Foldchange Foldchange D556C 1 1 1 D556T 3.5 3.4 3.7 Using https://www.biostars.org/p/7138/ prism when I graphically represent the fold change data I get error bars for the treated cell line, how do I get error bars for the untreated cell line to which the treated has been normalised? i.e all untreated replicates have a value of 1. When I look in the literature results are displayed with error bars on the untreated 1 x sample? Many thanks Dolores data • 9.2k views ADD COMMENT • link • Not following Follow via messages Follow via email Do not follow modified 5.5 years ago by Chris Evelo ♦ 9.7k • written 5.5 years ago by Dolores Hamilton • 0 This is not very clear at all. Are you saying that there are 7 cell lines, each either treated or untreated? It would help if you could show some raw data and explain what software was used. It also sounds as though you have 2 observations per cell line (1 treated, 1 untreated), in which case error bars are quite meaningless. ADD REPLY • link written 5.5 years ago by Neilfws ♦ 46k 1 5.5 years ago by Ch
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