Error In Solvent Extraction Procedures
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Request full-text Possible source of error in solvent extraction procedures for catecholaminesArticle in European Journal of Pharmacology 4(4):435-40 · December 1968 with 379 ReadsDOI: 10.1016/0014-2999(68)90031-9 · Source: PubMed1st Aaron H. Anton2nd David F. SayreAbstractEvidence has been presented that several naturally-occuring reducing agents may be the source of errors in solvent extraction procedures for endogenous catecholamines. By the use of the sulfhydryl inhibitor, p-chloromercuriphenyl sulfonic acid and carrying out the extraction with a pH 7.8 phosphate buffer, interference by the reducing agents was circumvented. This http://www.ncbi.nlm.nih.gov/pubmed/5724920 modified solvent extraction procedure was highly reproducible and gave the same norepinephrine, epinephrine, dopamine and dopa values as our Al2O3-trihydroxyindole procedure with a variety of tissues from several laboratory animals.Do you want to read the rest of this article?Request full-text CitationsCitations20ReferencesReferences5Estimation of catecholamines in the brains of embryonic and newly hatched chickens and the effects of https://www.researchgate.net/publication/17460987_Possible_source_of_error_in_solvent_extraction_procedures_for_catecholamines reserpine[Show abstract] [Hide abstract] ABSTRACT: Reserpine significantly decreased the concentrations of atecholamines (CA) in the brains of 14- and 18-day-old chicken embryos when administered prior to incubation in a dose that has little or no effect upon hatchability. Administration of this drug had profound effects upon the fluorometric estimation of CA in the brains of both embryonic and newly hatched chicks. This can lead to an erroneous interpretation of the quantitative effects of reserpine on CA concentrations in the brain.Article · Jan 1969 S B SparberF E ShidemanReadMeasurement of Catecholamines[Show abstract] [Hide abstract] ABSTRACT: Many drugs and other procedures produce all or part of their effects on smooth muscle indirectly, by modifying the release of catecholamines from adrenergic nerve terminals. (The loci and mechanisms of action of such drugs are discussed in Part II, Chapter 5) The estimation of the catecholamine contents of smooth muscles and of their perfusates or bathing media is thus frequently an important part of the study of drugs acting on
allUploadSign inJoinBooksAudiobooksComicsSheet Music Liquid-liquid Extraction PHARM 309 Liquid-liquid extraction is a versatile and dependableseparation technique wherein anaqueous https://www.scribd.com/doc/5552507/Liquid-Liquid-Extraction solutionisusually brought into contact with anotherorganicsolvent, exclusivelyimmisciblewith the former, so as to affect a legitimate and actual transfer of eitherone or more solutes into the latter.The separation technique is superior to others dueto e ase of use, f aster error in extraction times, d ecreasedvolumes of solvent, and their superior ability to c oncentrate the analytes. Introduction: Invariably such separations may be performed byshakingthe two liquids in aseparatory funnel(separating funnel)for a few minutes; and may beextended either to large error in solvent quantities of pharmaceutical substances or trace levels.Liquid-liquid extractions are usually accomplishedwith a separatory funnel. The two liquids are placedin the separatory funnel andshaken (moderately)toincrease the surface area between the phases.When the extraction is complete, the liquids areallowed to separate, with thedenser phasesettlingto the bottom of the separatory funnel. AboutBrowse booksSite directoryAbout ScribdMeet the teamOur blogJoin our team!Contact UsPartnersPublishersDevelopers / APILegalTermsPrivacyCopyrightSupportHelpFAQAccessibilityPressPurchase helpAdChoicesMembershipsJoin todayInvite FriendsGiftsCopyright © 2016 Scribd Inc. .Terms of service.Accessibility.Privacy.Mobile Site.Site Language: English中文EspañolالعربيةPortuguês日本語DeutschFrançaisTurkceРусский языкTiếng việtJęzyk polskiBahasa indonesiaLiquid Liquid Extraction by Apurba Sarker Apu10K viewsEmbedRelated interestsSolution, Solvent, Phase (Matter), EmulsionDownloadDescriptionLecture note on liquid liquid extractionLecture note on liquid liquid extractionRead on Scribd mobile: iPhone, iPad and Android.Copyright: Attribution Non-Commercial (BY-NC)Download as PPTX, PDF, TXT or read online