Human Error In Pipetting
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Why Should Mouth Pipetting Be Avoided
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high when it appears to align with the mark.
What Is Nss Chemically
It you are looking down at the pipet, the meniscus will be too low when it appears http://www.ttelaboratories.com/Accuracy-Matters-Blog/entryid/25/10-ways-to-prevent-pipetting-errors to align with the mark. Forcing the solution out of the pipet causes too much to be delivered. Using a dirty pipet causes too little or contaminated solution to be delivered. Leaving little droplets http://chemed.chem.purdue.edu/genchem/lab/equipment/pipet/error.html behind on the walls (except for the small amount in the tip) causes too little solution to be delivered. Forcing the pipet into the bulb usually causes liquid to be sucked into the bulb. This contaminates the bulb which must then be thoroughly rinsed and dried. Allowing the tip of the pipet to rise above the liquid in the container usually causes the liquid to be sucked into the pipet bulb. A broken or chipped pipet can reduce the amount of liquid held after transfer. This causes too much liquid to be delivered. Pipet Menu Laboratory Information General Chemistry Help Homepage
be standardized. However, liquid handling quality assurance programs commonly focus on pipette calibration, repair and maintenance but http://www.laboratoryequipment.com/article/2009/08/operators-sources-error rarely include protocols for verifying operator technique. Yet just as malfunctioning pipettes and automated liquid handlers can result in inaccurate delivery volumes and alter laboratory results, so too can poor pipetting skills. The urgent need for operator training is discussed in a recently distributed letter from the Centers for Medicare and Medicaid error in Services (CMMS), which cites “poor pipetting technique” as one of the causes of “questionable test results” generated with an HIV test kit. After the erroneous results were reported, a root cause analysis determined that the operator incorrectly used the pipetting system during the test protocol, which required the addition of a why should mouth micro specimen of sample. Following this incident, the Center’s Department of Health and Human Services formally recommended that labs institute staff training, competency assessments and the evaluation of quality assurance protocols to prevent similar error. Like baseball players being coached on swinging a bat and new drivers taking lessons before being able to operate a car legally, lab technicians must be trained on proper pipetting skills. Technique must be standardized within and across labs to minimize error and facilitate data comparability. Otherwise data integrity may be at risk. The following discusses common pipetting technique errors, their impact on pipetting accuracy and precision, and how effective training programs can minimize the risk of error. Operator technique and errors Researchers pipette on a daily basis, but many have never received formal training on pipetting. And like most repetitive, common tasks, pipetting is often taken for granted. Understanding the steps researchers can take to improve pipet
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