Micropipetting Error
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& Online ToolsTaming the LiteratureWriting, Publishing & Presenting- View all of these channels -Webinar Festival Get Social: Biotix BiotixLog InHome 30218 17 Ways to Stop Pipetting Errors Ruining Your Experiments By Biotix - 15th September, 2016 If you work at the bench, accurate pipetting is crucial. Without accurate pipetting, your experiments would not be reproducible, your stock solutions would be inaccurate, and your assays would common pipetting errors have such large errors that comparing them would be meaningless. But luckily, there's no need to worry—your trusty, precision micropipettes take care of that for you. Or do they? Precision instruments they may be, but the accuracy of your micropipettes depends on you. You need to maintain your pipettes, practice good technique, and have an understanding of how they work. Only once you have mastered those aspects can you claim to have anything like precision instruments at your disposal. Here's how: Know How Your Pipette Works 1. You Use an Air Displacement Pipette, Most Likely An air displacement pipette works a bit like a syringe, except that there is an air-filled cushion between the piston and the sample. The air cushion prevents the piston from coming into contact with the solutions. Keeping the solution and the pipette barrel apart is good, but it also puts some limitations on the pipette. Temperature and pressure affects the volume of the air cushion, which affects pipetting accuracy. Also, volatile solvents can evaporate into the air cushion, which leads to an inaccurate and lower dispensed volume than what is displayed on the pipette. The barrel of air displacement pi
microliter (10-6 liter, 0.000001), or one millionth of a liter. Small errors in pipetting can translate into ENORMOUS errors in experiments. This is due to the fact that solutions you are pipetting are at high concentrations. Inaccurate pipette error margin pipetting is a chief contributor to poor laboratory results. Take the following precautions when
Pipette Error Value
micropipetting: -Never use the micropipettor without the tip in place; this could ruin the piston. -Never rotate the volume
Proper Pipetting Technique
adjustor beyond the upper or lower range of the pipette, as stated by the manufacturer. -Never invert or lay the micropipettor down with a filled tip; fluid could run back into the piston. http://bitesizebio.com/344/17-ways-to-stop-pipetting-errors-ruining-your-experiments/ -Never let the plunger snap back after withdrawing or expelling fluid; this could damage the piston. Instead gently and slowly draw up the liquid into the pipette tip. -Never immerse the barrel of the micropipettor in fluid. -Never flame the tip of the micropipettor. -Never reuse a tip that has been used to measure a different reagent.
There are essentially three sizes of micropipettors based upon volume ranges: http://www.berwicksclasses.org/AP%20Biology/Biology%20Assignments/Micropipetting%20Lab.htm 2-20µl, 20-200µl, 200-1000µl. Typically the same size tips are used for the small and mid-range volumes with larger tips being necessary for the larger sized micropipettor. Exercise I Parts of a micropipette: Identify the following parts of a micropipette- a. Plunger button b. Tip ejector button c. Volume adjustment dial d. Digital volume indicator e. Shaft f. Attachment point for a disposable tip Exercise II Using the Micropipette: Two “stops” when ejecting liquids. Depressing to the first stop measures the desired volume. Depressing to the second stop introduces an additional volume of air to blow out any solution remaining in the tip. Notice these friction stops; they can be felt with the thumb by pushing on the plunger. When drawing liquid into the pipette only go to the FIRST stop; going beyond this initial stop will introduce error into your pipetting and your experiments. Withdraw 10µl of colored solution from container: a. Depress the plunger to first stop and hold it in this position. Dip the tip into the solution to be pipetted, and draw fluid into the tip by gradually releasing the plunger. Be sure that the tip remains in the solution while you are releasing the plunger. b. Slide the piVerification System --ArtelWare -PCS Pipette Calibration System --PCS Software -VMS Volume Measurement System -Training & Seminars --Pipette Quality Management Certification --Pipetting Proficiency Certification Resource http://www.artel-usa.com/resource-library/pipetting-technique-as-source-of-error/ Library -Common Errors In Liquid Handling -DMSO & Custom Reagent Volume Verification -Environmental Influences on Accuracy and Precision -Performance Verification of Automated Liquid Handlers -Method/Protocol Optimization with Automated Liquid Handlers -Measure Mixing Efficiency -Pipette Calibration -Pipetting Technique -Regulatory & Standards Events -Conferences -Pipetting Challenge --Upcoming Events --Past Events -Training & error in Seminars Digest -Ask An Expert -Case Study -Feature Article -Regulatory Update -Technical Resource Support & Ordering -Ordering -PCS Support -MVS Support -Warranty & Service Contact Us Follow Us Company Accreditation & Compliance Quality Leadership Press Room Career Opportunities Current Openings Internship Program Community Products & Services Artel Technology Artel Liquid Handling sources of error Service MVS Multichannel Verification System ArtelWare PCS Pipette Calibration System PCS Software VMS Volume Measurement System Training & Seminars Pipette Quality Management Certification Pipetting Proficiency Certification Resource Library Common Errors In Liquid Handling DMSO & Custom Reagent Volume Verification Environmental Influences on Accuracy and Precision Performance Verification of Automated Liquid Handlers Method/Protocol Optimization with Automated Liquid Handlers Measure Mixing Efficiency Pipette Calibration Pipetting Technique Regulatory & Standards Events Conferences Pipetting Challenge Upcoming Events Past Events Training & Seminars Digest Ask An Expert Case Study Feature Article Regulatory Update Technical Resource Support & Ordering Ordering PCS Support MVS Support Warranty & Service Media Types 10 Tips Application Note Case Study Lab Report Poster Presentation Publication Topics Common Errors In Liquid Handling DMSO & Custom Reagent Volume Verification Environmental Influences on Accuracy and Precision Method/Protocol Optimization with Automated Liquid Handlers Measure Mixing Efficiency Pipette Calibration Pipetting Technique Regulatory & Standards Minimi
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