Parallax Error Pipette
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are the most common errors in pipetting technique?UpdateCancelAnswer Wiki5 Answers Giridhar Sekar, PhD. in Biochemistry.Written 93w agoOthers have covered micropipets. Let me cover graduated pipets and volumetric pipets (you know those glass things). Oh yes, you can make mistakes when using those as well.Edit : List item 1 has sources of error in micropipetting been corrected based on some valuable education from Tim Wiryaman.1. The first thing is to be what common error in handling a micropipette aware whether the last drop in your pipet is to be blown away or not. Some pipets are designated as TD (to deliver) others are
Why Should Mouth Pipetting Be Avoided
designated as TC (to contain). In a TD pipet, the last drop is not meant to be shaken or "blown" away. These pipets are calibrated to deliver the correct volume of liquid without having to blow it away. In a TC
Why Should Mouth Pipetting Be Avoided In The Laboratory
pipet, the pipets are calibrated to contain the correct volume. In this case, the last drop needs to be blown away. Many serological pipets are TC pipets and the last drop should be blown away. These pipets are marked with 2 rings on the top of the pipet. (P.S. - I mention "blow" away, but mouth pipeting has been done away with in most laboratories, so the equivalent would be to use the bulb or the mechanical aspirator to remove the last pipette error margin drop.)2. Parallax errors - This is common among students both when reading pipets and burets. The pipet has to be held up to eye level, or operator has to safely bend down to read the pipet at eye level. "Lazy" reading of pipets can lead to large changes in pipeted volumes. 3. The conundrum of the meniscus. - For some reason, this seems to confuse people. But the simplest way to do this is, if you can see the lower meniscus then use that. If you cannot see the lower meniscus, read the upper meniscus. Do not guess where the lower meniscus might be. The confusion arises from the instruction I have heard some people restate "Use the lower meniscus for clear liquids and upper meniscus for coloured liquids." The instruction is misleading when you use light coloured liquids where the lower meniscus is visible. All pipets are calibrated to the lower meniscus, so use it as long as you can see it. If the solution is very dark or opaque to the extent that the lower meniscus is not visible, only then use the upper meniscus. In the case of liquids like mercury that form a convex meniscus, use the top of the convex meniscus. 4. Using the correct size of pipets - I would often see students use a 10 mL graduated pipet to measure out 1 mL liquid when a perfectly usable 2 mL pipet is readily available for them to use
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Pipette Error Value
Lab Skills: Volumetric Lab Equipment Parallax difference between volumetric pipette and ostwald folin pipette Error Parallax is a deceptive change in the relative position of an object with a source of random error in using a volumetric flask change in the position of the observer. In the context of reading a piece of volumetric glassware, such as a measuring cylinder, burette, or volumetric https://www.quora.com/What-are-the-most-common-errors-in-pipetting-technique flask, the meniscus should be at eye level otherwise there will be an error in the reading. If the meniscus is above eye level an increased volume measurement will be made, conversely if the eye is above the meniscus then a lower volume reading will be made. Parallax Error Parallax http://www.chromacademy.com/lms/sco679/05-Parallax-Error.html?fChannel=22&fCourse=97&fSco=679&fPath=sco679/05-Parallax-Error.html is a deceptive change in the relative position of an object with a change in the position of the observer. In the context of reading a piece of volumetric glassware, such as a measuring cylinder, burette, or volumetric flask, the meniscus should be at eye level otherwise there will be an error in the reading. If the meniscus is above eye level an increased volume measurement will be made, conversely if the eye is above the meniscus then a lower volume reading will be made. Parallax error can also be applied to reading volume dials of micropipettes. The dial should be at eye level and directly in front of the user. Home | About Us |Contact Us | Subscribe |Terms and Conditions | Advertise | Privacy Policy Login Free Lite Membership Become a Premium Member Get an Agilent Sponsored Academic License Contact us
Celebrations Home & Garden Math Pets & Animals Science Sports & Active Lifestyle Technology Vehicles World View www.reference.com Science Chemistry Chem Lab Q: What is parallax error in chemistry? A: Quick Answer In chemistry, a parallax error is a deceptive shift in https://www.reference.com/science/parallax-error-chemistry-f7e3da1cff0fddcd an object's actual position due to personal perception. This type of error commonly occurs in https://books.google.nl/books?id=s_i8oPwYLWEC&pg=PA3&lpg=PA3&dq=parallax+error+pipette&source=bl&ots=nYhwj2ltEK&sig=L4wE5qIAnQy8_9iL9Gt9E7uYMEQ&hl=en&sa=X&ved=0ahUKEwjZs9fJh-bPAhXC0xoKHc0kC7kQ6AEIazAO a laboratory setting with the use of specialized measuring instruments called volumetric glassware. Continue Reading Keep Learning What is a parallax error? Why were ancient people unable to detect a stellar parallax? What are some sources of error in synthesis of alum from aluminum foil? Full Answer A meniscus refers to the crescent-shaped upper surface of a liquid placed inside volumetric error in glassware, such as a pipette, buret or graduated cylinder. In reading the volume of the liquid, the observer should be at eye level with the meniscus. Otherwise, a parallax error occurs.The reading is higher than the actual value when the observer's eye is below the meniscus. Conversely, a reading that is lower than the actual value results when the marking is viewed from an angle that is higher than the meniscus. Learn more about Chem Lab why should mouth Sources: centre.edu southeastern.edu dartmouth.edu Related Questions Q: How do you calculate relative error? A: To calculate relative error, you must first calculate absolute error. Absolute error is the quantitative amount of incorrectness between an estimate and th... Full Answer > Filed Under: Chem Lab Q: What is a conical flask? A: Also known as an Erlenmeyer flask, a conical flask is a glass vessel used in chemistry laboratories, and it has a wide flat bottom that tapers up into a na... Full Answer > Filed Under: Chem Lab Q: Why do scientists use the Kelvin scale? A: Scientists use the Kelvin temperature scale because it makes solving physics, astronomy and chemistry equations easier, since it lacks negative numbers. Ze... Full Answer > Filed Under: Chem Lab Q: What is an organic chemistry model kit? A: An organic chemistry model kit is a set of movable components, usually plastic, which students use to build and manipulate models of molecules. These sets ... Full Answer > Filed Under: Chem Lab You May Also Like Q: What is pK in chemistry? Q: What is a pipestem triangle used for? Q: What is error propagation? Q: What is an esterification lab? Q: What are some methods for fixing error 0x80004005 in Windows? Q: What happens when an enzyme is denatured? PEOPLE SEARCH FOR Definition of Parallax Error Meaning of Parallax
van GoogleInloggenVerborgen veldenBoekenbooks.google.nlhttps://books.google.nl/books/about/Chemistry_Expression.html?hl=nl&id=s_i8oPwYLWEC&utm_source=gb-gplus-shareChemistry ExpressionMijn bibliotheekHelpGeavanceerd zoeken naar boekenGedrukt boek aanschaffenGeen eBoek beschikbaarPanpac Education Pte LtdBol.comProxis.nlselexyz.nlVan StockumZoeken in een bibliotheekAlle verkopers»Boeken kopen Google PlayBrowse door 's werelds grootste eBoekenwinkel en begin vandaag nog met lezen op internet, je tablet, telefoon of eReader.Ga nu naar Google Play »Chemistry Expression: An Inquiry Approach : O Level Science, ChemistryPanpac Education Pte Ltd, 2007 - 333 pagina's 0 Recensieshttps://books.google.nl/books/about/Chemistry_Expression.html?hl=nl&id=s_i8oPwYLWEC Voorbeeld weergeven » Wat mensen zeggen-Een recensie schrijvenWe hebben geen recensies gevonden op de gebruikelijke plaatsen.Geselecteerde pagina'sTitelbladInhoudsopgaveIndexInhoudsopgaveMeasurement in Chemistry 1 Methods of Purification and Analysis 14 The Three States of Matter 31 Elements Mixtures and Compounds 46 Atomic Structure 64 Chemical Bonding 78 Relative Masses of Atoms and Molecules 88 The Mole 96 Redox Reactions 208 The Periodic Table 224 Metals 240 Extraction of Metals 251 Air 266 The Hydrocarbons 284 Alcohols and Carboxylic Acids 304 IT Links 319 MeerAcids Bases and Salts 118 Qualitative Analysis 149 Energy Changes 166 Energy from Fossil Fuels 173 Speed of Chemical Reactions 185 Data Table 323 Glossary 327 Index 331 Copyright MinderOverige edities - Alles weergevenChemistry Expression: An Inquiry Approach : O Level, Special, ExpressGedeeltelijke weergave - 2006Veelvoorkomende woorden en zinsdelenalcohol alkali alkanes alkenes aluminium ammonia ammonium aqueous boiling point bromine burns calcium carbonate called carbon atoms carbon dioxide carbon monoxide chemical equation chemical reaction colour colourless combustion concentration contains copper(II copper(ll crystals dissolved electrons elements ethanoic acid ethanol ethene example exothermic formula fossil fuels fractional distillation gases graph heat energy homologous series hydrocarbons hydrochloric acid hydrogen hydrogen atom insoluble ionic compounds ions iron iron(III liquid litmus paper