Random Error In Spectrophotometry
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it is up to you to know what this range is. The instrument will cheerfully inform you that the concentration is "7.112 mg/L" on its digital display sources of error in spectrophotometry lab or in a computer-generated report, when the instrumental error is actually sources of error in absorption spectroscopy approaching 10%. For spectrophotometric errors, consider the following model instrument: The monochromator splits the white light of the
Spectrophotometer Error Range
source into it component wavelengths, and allows a particular band of wavelengths to pass through the sample. Light of power Po goes into the cell, and light of power
Error In Absorbance
P comes out. The light is converted into electrical current in the Detector, and transformed into Absorbance in the readout. Absorbance is related to concentration using Beer's Law, A=abC. The equations are: A is the Absorbance, C is the concentration, a is the absorptivity and b is the cell path length. If C is in mg/L and b sources of error in absorbance spectroscopy in cm, then a will have the units cm-1mg-1L. Our instrument measures P and Po with an uncertainty determined by the stability of the light source and the electronics. As %T approaches 100% and A approaches zero, the uncertainties in measuring %T dominate the error in A. It can be shown that the absolute error in A due to %T error = Error in %T*0.434/%T. [For example, in a Spectronic 20 the instrumental noise is 0.5%T. At an Absorbance of 0.100 (%T=79.4%) the error is 0.5%T*0.434/79.4%T=0.003 A, a 3% error in A.] In simpler terms, at low concentrations the instrument can no longer "see" the color present with any accuracy. As %T approaches zero and A approaches infinity, we run out of light to measure when the concentrations become too high. In addition to the random errors inherent in measuring very low light levels, Stray Light limits our ability to measure highly absorbing solutions. All spectrophotometric instruments allow some of the light to bypass the cell and add to the light reachi
constants depend on the magnitude of systematic and random errors respectively. Good accuracy requires that systematic errors be reduced as far as possible. The use of analytical grade reagents will reduce errors due to purity of reagents
Spectrometer Errors
such as acid or alkali and the salt used for ionic background. Errors in spectrophotometer lab report temperature control are systematic errors. Electrode calibration error is also a source of systematic error, of particular importance when comparing duplicate systematic errors titration curves. Good precision requires that random errors be reduced as far as possible. All instrumental measurements are subject to random error. The magnitude of this error is instrument specific and, in the case of http://zimmer.csufresno.edu/~davidz/Chem102/Gallery/Spectro/SpectroPhot.html spectrophotometric measurements is also dependent on the magnitude of the measured quantity. The objective of the stability constant refinement is to calculate values that correspond to experimental observations within experimental error. This means that estimates are needed of the random errors present in the experimental measurements. Potentiometry Two error estimates are required by Hyperquad for potentiometric titration data. Error in titre volume. The error in titre volume can be estimated by http://www.hyperquad.co.uk/step_by_step/exp1.htm weighing. It is a good idea to check both the accuracy and precision of a burette. If the weight delivered at a given temperature is measures for a series of volumes the data can be fitted to a straight line; the required error value will then be given by the error on the slope. Error in electrode reading. The error in electrode reading is more difficult to estimate. It is common practice to assume a value based on personal observations of the volt meter or pH meter. In Hyperquad it is assumed that the electrode error is a constant, independent of the actual value. Spectrophotometry A potential source of systematic error is small differences of baseline between different spectra. In order to minimize baseline errors it is preferable that neither sample nor reference cell should be moved between measurements of spectra. In practice this means either using a flow-cell or a fibre-optic probe or building a titration cell for a particular spectrophotometer. If measurements are to be made in alkaline solutions then the necessity of excluding atmospheric CO2 indicates that a closed titration system must be used. Baseline error is also affected by whether the spectrophotometer is a single- or double-beam device. Instruments based on diode-array detectors are usually si
15:00 SGT/ 21:00- 03:00 EDT. Apologies for the inconvenience. Remove maintenance message Skip to main content Log in / Register Advertisement Go to old article view Advertisement http://onlinelibrary.wiley.com/doi/10.1002/col.5080160605/abstract Color Research & Application Explore this journal > Previous article in issue: Back to Helmholtz Previous article in issue: Back to Helmholtz Next article in issue: Efficient uniform illuminators using diffusing optics Next article in issue: Efficient uniform illuminators using diffusing optics View issue TOC Volume 16, Issue 6 December 1991 Pages 360–367 ArticlePropagation of random errors in spectrophotometric colorimetryAuthorsMark D. Fairchild,Munsell Color error in Science Laboratory Center for Imaging Science Rochester Institute of Technology One Lomb Memorial Drive Rochester, New York 14623-0887Search for more papers by this authorLisa ReniffMunsell Color Science Laboratory Center for Imaging Science Rochester Institute of Technology One Lomb Memorial Drive Rochester, New York 14623-0887Search for more papers by this authorFirst published: December 1991Full publication historyDOI: 10.1002/col.5080160605View/save citationCited by: 10 articles Citation tools Set citation sources of error alert Check for new citations Citing literature AbstractEvery physical quantity with an a priori range of numerical values constituting a continuum is subject to error in its measurement. It is important to report the highest amount by which any measured quantity might be in error. Optical radiation measurements are typiclly based on determination of the ratio of the instrumental reading of a calibrated standard to the instrumental reading of the test sample. There are random and systematic errors in both the instrumental readings and the calibration values. The uncertainty in a calculated value due to random error in its constituents can be determined using well known techniques of error propagation. Examples of error propagation through spectral reflectance factor measurements and colorimetric calculations are presented. The standard deviations of CIELAB coordinates for typical measurements can be as high as 0.258 due only to random errors in the calibration chain. Continue reading full article Enhanced PDFStandard PDF (765.1 KB) AncillaryArticle InformationDOI10.1002/col.5080160605View/save citationFormat AvailableFull text: PDFCopyright © 1991 Wiley Periodicals, Inc., A Wiley Company Request Permissions Publication HistoryIssue online: 13 March 2007Version of record online: 13 March 2007Manuscript Accepted: 15 April 1991Ma
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