Flx800 Error Codes
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Biotek Elx808 Manual
Questions Absorbance Readers How can we verify that the measurements above the OD accuracy, linearity biotek elx800 manual and repeatability specification are acceptable for our lab? First run the Absorbance Test Plate and, in addition to the software calculated OD values,
Biotek Error Codes
manually calculate the value over 2.500 OD for the wavelength of interest. Next run a liquid test where the higher values are closer to the sample values that you want to use. The accuracy of the lower values biotek plate reader elx808 can be checked against the Absorbance Test Plate values and the accuracy/linearity for higher OD values can be extrapolated based on the curve fit of the test dye intercept at zero OD. Why is the OD accuracy, linearity and precision specified for a smaller range than the defined dynamic range? The instrument has a range of operation from 0.000 to 4.000 OD, but the specifications for accuracy, linearity and reproducibility only go to 2.500 OD. The range biotek gen5 manual allows the user to make measurements above 2.500 OD and roughly determine how much the sample needs to be diluted if a very accurate value is required. The accuracy of values over 2.500 OD is limited as described below. (Note that some cuvette based spectrophotometers are only specified for accuracy to 1.0 OD, but can read up to 5.0 OD.) Microplate readers, like many other optical electronic instruments, have varying accuracy, repeatability, and linearity specifications that must become less stringent as higher OD values are approached. This is due to the nature of the reader’s optical and electronic sensing circuits. Assuming that a typical instrument is capable of parsing or dividing the dynamic signal range into exactly 60,000 “counts” then the following holds true: 0 OD is 100% light (60,000 counts of signal), 1 OD is 10% light (6,000 counts of signal), 2 OD is 1% light (600 counts of signal), 3 OD is 0.1% light (60 counts of signal), and 4 OD is down to 0.01% of full light (6 counts of signal). Thus at very high ODs a variation of 0.1 OD is only a count of signal or less. This and other factors (such as bulb strength, ambient blocking etc.) do not allow the readers to always have the best results above a specific value. Samples that are very dark should be diluted in order t
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