Real Time Pcr Error Propagation
fold-change data? I'm considering some real time data on tissues treated and untreated upon a given stress. Obviously based on three technical replicate in each condition I obtained a normalized expression value associated with a standard error based on the replicates. If I calculate the log2 of the fold change (treated / untreated) how can I calculate the corresponding error bars? Topics Basic Statistical Methods × 401 Questions 93 Followers Follow Real-Time PCR × 2,153 Questions 3,371 Followers Follow Transcriptomics × 397 Questions 17,826 Followers Follow Gene Expression × 1,722 Questions 25,298 Followers Follow Apr 17, 2014 Share Facebook Twitter LinkedIn Google+ 0 / 0 Popular Answers Jochen Wilhelm · Justus-Liebig-Universität Gießen I suppose you have a standard error of the mean log expression for each condition se_A and se_B. The log ratio is the difference between the log expressions, and the standard error of this difference is given by sqrt(se_A²+se_B²)*. *assuming A and B are uncorrelated See: http://en.wikipedia.org/wiki/Propagation_of_uncertainty Apr 17, 2014 All Answers (7) Jochen Wilhelm · Justus-Liebig-Universität Gießen I suppose you have a standard error of the mean log expression for each condition se_A and se_B. The log ratio is the difference between the log expressions, and the standard error of this difference is given by sqrt(se_A²+se_B²)*. *assuming A and B are uncorrelated See: http://en.wikipedia.org/wiki/Propagation_of_uncertainty Apr 17, 2014 Jo Vandesompele · Ghent University All formulas for error propagation during qPCR data-analysis are mentioned in attached paper. The formulas are integrated in Biogazelle's qbase+ software (http://www.qbaseplus.com). Source Available from: Jo Vandesompele Article: Hellemans J, Mortier GR, De Paepe A, Speleman F, Vandesompele JqBase relative quantification
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- (May/06/2011 )Visit this topic in live forum Printer Friendly VersionHi all, I'm no mathematician, but I know that the error from my control set and real time from my experimental set should be reflected in final error bars. 1. What is the appropriate formula to use (there seems to be more than one way to look at real time pcr this) 2. How do you tell excel to do it? Thanks for any help. -thumbclaw- all the necessary formulas for the proper error propagation can be found in this paper: Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J. qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol. 2007;8(2):R19. PubMed PMID: 17291332 full text is free. -tea-test- Visit this topic in BioForum Printer Friendly Version About Terms of Service Privacy Feedback Sponsorship © 1999-2013 Protocol Online, All rights reserved.
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